Midfielders Gavi and Isco were recalled on Monday by Spain manager Luis de la Fuente in his team for the semi -final of next month's Nations League against France.
Gavi van Barcelona, 20, played for the last time for Spain in November 2023 before he was sidelined for a few months after tearing his knee latters.
Gavi, closed 27 times by Spain, has made 42 performances for Barcelona this season and won Laliga, the Copa del Rey and Supercup with the Catalan club.
After an absence of six years, Isco returned to the Spain team.
The 33-year-old former Real Madrid player has been rewarded for a good season with real betis in which he scored 12 goals and gave 10 assists in 32 performances and helped them to reach the final of Wednesday's conference.
Spain will play France in the German city of Stuttgart on 5 June.
Team
Goalkeepers: Unai Simon, David Raya, Alex Remiro
Defenders: Pedro Porro, Oscar Mingueza, Robin Le Normand, Pau Cubarsi, Dean Huijsen, Alejandro Grimaldo, Marc Cucurella, Dani Vivian
Midfielders: Martin Zubimendi, Fabian Ruiz, Mikel Merino, Alex Baena, Pedri, Fermin Lopez, Gavi, Isco
Forward: Alvaro Morata, Lamine Yamal, Nico Williams, Yeremy Pino, Dani Olmo, Samu Aghowa, Mikel Oyarzabal.